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| Catalog Code: | NB120-5464 |
| Product Name: | VEGF Receptor 2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-VEGF Receptor 2 [Tyr951] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.4 mg/ml |
| Immunogen: | Synthetic phospho peptide (Human)containing Tyrosine 951. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (72% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.25 - 1 ug/ml. Detects a band of approximately 200 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Background: | VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-CD309 antigen antibody, anti-Fetal liver kinase 1 antibody, anti-FLK1 antibody, anti-KDR antibody, anti-Kinase insert domain receptor antibody, anti-KRD1 antibody, anti-Ly73 antibody, anti-Protein tyrosine kinase receptor FLK1 antibody, anti-Vascular endothelial growth factor receptor 2 antibody, anti-VEGFR antibody, anti-VEGFR2 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Tyr951 |
| General References: | General / background references:Zeng H et al. Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2 (KDR) are essential for vascular permeability factor/vascular endothelial growth factor-induced endothelium migration and proliferation, respectively. J Biol Chem 276:32714-9 (2001). Rahimi N et al. Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells. J Biol Chem 275:16986-92 (2000). Shalaby F et al. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376:62-6 (1995). |
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