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| Catalog Code: | NB120-5455 |
| Product Name: | Hsp90 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp90 (3B6) |
| Clone: | 3B6 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Purified mouse Hsp 90. |
| Specificity: | Detects heat shock protein 90 kDa (Hsp 90) from human, mouse and rat tissues. This antibody does not detect chicken Hsp 90. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | WB: Use at a dilution of 1/500. Detects a band of approximately 90 kDa representing Hsp 90 from MCF7 cell extract. ab5455 does not immunoprecipitate Hsp 90 in any form (free or complexed).Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Ascites |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:500 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Heat Shock Protein 90 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% Sodium Azide |
| General References: | <b>General / background references:</b>Meng X <i>et al.</i> Mutational analysis of Hsp90 alpha dimerization and subcellular localization: dimer disruption does not impede "in vivo' interaction with estrogen receptor. <i>J Cell Sci</i> <b>109 ( Pt 7)</b>:1677-87 (1996). <PUBMED:8832390>Nimmesgern E & Hartl FU ATP-dependent protein refolding activity in reticulocyte lysate. Evidence for the participation of different chaperone components. <i>FEBS Lett</i> <b>331</b>:25-30 (1993). <PUBMED:8104824>Matts RL & Hurst R Evidence for the association of the heme-regulated eIF-2 alpha kinase with the 90-kDa heat shock protein in rabbit reticulocyte lysate in situ. <i>J Biol Chem</i> <b>264</b>:15542-7 (1989). <PUBMED:2768277> |
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