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| Catalog Code: | NB120-5444 |
| Product Name: | Hsp70 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp70 (4G4) |
| Clone: | 4G4 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Human recombinant Hsp 70 over expressed in E. coli. |
| Epitope: | Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human Hsp 70, a region that has been shown to be involved in stress-induced nucleolar localization. |
| Specificity: | ab5444 detects heat shock protein 70 kDa (Hsp 70) from human tissues. This antibody specifically detects Hsp 70 and does not cross-react with other members of the Hsp 70 family. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.05 ml purified Mouse ascites. |
| Uses: | WB: Use at a dilution of 1/5000. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Ascites |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:5,000 |
| Species Summary: | Hu |
| Alternate Names: | anti-DAQB 147D11.1 001 antibody, anti-Heat shock 70 kDa protein 1 antibody, anti-heat shock 70kDa protein 1A antibody, anti-Heat shock 70kDa protein 1B antibody, anti-heat shock protein 70 antibody, anti-HSP70 1 antibody, anti-HSP70 2 antibody, anti-HSP70.1 antibody, anti-HSP72 antibody, anti-HSPA1 antibody, anti-HSPA1A antibody, anti-HSPA1B antibody, anti-XXbac BCX40G17.3 001 antibody |
| Package Size: | 0.05 ml |
| General References: | General / background references:Carr VM et al. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol 348:150-60 (1994). Milarski KL et al. Cell cycle-dependent association of HSP70 with specific cellular proteins. J Cell Biol 108:413-23 (1989). |
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