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| Catalog Code: | NB120-5443 |
| Product Name: | PDGFR Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PDGFR [Tyr572/Tyr574] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic phospho peptide, containing Tyrosine 572 and 574 of Human PDGFR alpha. This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog. |
| Cross Reactivity: | Cross-reacts with Mouse.Expected to cross-react with Human (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.35 - 1 ug/ml. Detects a band of approximately 170 - 185 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Background: | Platelet-derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation. PDGFR alpha tyrosines 572 and 574 (579 and 581 in PDGFR beta) are autophosphorylated in the activated receptor, and bind and activate Src family kinases. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Mu, |
| Alternate Names: | anti-Alpha platelet derived growth factor receptor antibody, anti-Beta platelet derived growth factor receptor antibody, anti-CD 140A antibody, anti-CD 140b antibody, anti-CD140A antibody, anti-CD140a antigen antibody, anti-CD140b antibody, anti-CD140b antigen antibody, anti-Cluster of differentiation 140b antibody, anti-JTK 12 antibody, anti-JTK12 antibody, anti-MGC74795 antibody, anti-PDGF R alpha antibody, anti-PDGF R beta antibody, anti-PDGFR 1 antibody, anti-PDGFR 2 antibody, anti-PDGFR A antibody, anti-PDGFR alpha antibody, anti-PDGFR antibody, anti-PDGFR B antibody, anti-PDGFR beta antibody, anti-PDGFR1 antibody, anti-PDGFR2 antibody, anti-PDGFRA antibody, anti-PDGFRA protein antibody, anti-PDGFRB antibody, anti-Platelet derived growth factor receptor alpha antibody, anti-Platelet derived growth factor receptor alpha polypeptide antibody, anti-Platelet derived growth factor receptor beta antibody, anti-Platelet derived growth factor receptor beta polypeptide antibody, anti-Rhe PDGFRA antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Tyr572/Tyr574 |
| General References: | General / background references:Rolny C et al. Heparin amplifies platelet-derived growth factor (PDGF)- BB-induced PDGF alpha -receptor but not PDGF beta -receptor tyrosine phosphorylation in heparan sulfate-deficient cells. Effects on signal transduction and biological responses. J Biol Chem 277:19315-21 (2002). Carloni V et al. Cell adhesion regulates platelet-derived growth factor-induced MAP kinase and PI-3 kinase activation in stellate cells. Hepatology 36:582-91 (2002). Matsumoto T et al. Platelet-derived growth factor activates p38 mitogen-activated protein kinase through a Ras-dependent pathway that is important for actin reorganization and cell migration. J Biol Chem 274:13954-60 (1999). Hooshmand-Rad R et al. PDGF alpha-receptor mediated cellular responses are not dependent on Src family kinases in endothelial cells. J Cell Sci 111 ( Pt 5):607-14 (1998). Heldin CH et al. Signal transduction via platelet-derived growth factor receptors. Biochim Biophys Acta 1378:F79-113 (1998). |
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