| CatalogCode: | NB120-5442 |
| ProductName: | Hsp70 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp70 (2A4) |
| Clone: | 2A4 |
| Clonality: | Monoclonal |
| Immunogen: | Human recombinant Hsp 70 over expressed in E. coli. |
| Epitope: | Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human Hsp 70. |
| Specificity: | NB 120-5442 detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70 and, following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. Detects a band of approximately 70-72 kDa representing different members of the Hsp 70 family. |
| CrossReactivity: | Cross-reacts with Chicken, Drosophilia melanogaster, Fish, Human, Mouse, Rat, S. cerevisiae and Amphibians. Not yet tested in other species. |
| Packaging: | 0.05 ml Ascites Mouse ascites. |
| Uses: | IHC-P: Antigen retrieval is not essential but may optimise staining. Two-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | HeLa |
| Background: | The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria. |
| Storage: | Aliquot and store at -20C. |
| Purity: | Ascites |
| Isotype: | IgM |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | IF, WB, IHC-P |
| SpeciesSummary: | Dr, Hu, Mu, Rt, Ye, Am, Av, Fi |
| ALTnames: | anti-Heat Shock 70kDa protein antibody |
| ProteinTarget: | Hsp70 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Carr VM et al. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol 348:150-60 (1994). Milarski KL et al. Cell cycle-dependent association of HSP70 with specific cellular proteins. J Cell Biol 108:413-23 (1989). |
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