| CatalogCode: | | NB120-5441 |
| ProductName: | | Calpain 1 Antibody |
| Product Description: | | Mouse Monoclonal anti-Calpain 1 (2H2A7C2) |
| Clone: | | 2H2A7C2 |
| Clonality: | | Monoclonal |
| Immunogen: | | Purified bovine skeletal muscle 80 kDa mu-calpain subunit. |
| Epitope: | | This antibody recognizes an epitope between amino acids 245-265 (domain II) of human mu-calpain. |
| Specificity: | | This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. |
| CrossReactivity: | | Cross-reacts with Cow, Human, Pig and Rat.Not yet tested in other species. |
| Packaging: | | 0.1 ml |
| Uses: | | ICC: Use at a dilution of 1/25. WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in porcine LLC-PK1 cells with ab5441 results in diffuse cytoplasmic staining. Is unsuitable for IP.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic; Translocates to the plasma membrane upon Ca(2+) binding. |
| Control: | | Mouse lung lysate. |
| Background: | | The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with 50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation. |
| Purity: | | Ascites |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | Preservative: 0.05% Sodium AzideConstituents: Raw Ascites |
| ListPrice: | | 330 |
| AppSummary: | | ICC, WB |
| SpeciesSummary: | | Bv, Hu, Po, Rt |
| ProteinTarget: | | Calpain 1 |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | General / background references:Cong J et al. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem 268:25740-7 (1993). Lane RD et al. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res 203:5-16 (1992). |
| more information: | | company product webpage |