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| Catalog Code: | NB120-5392 |
| Product Name: | MAP 2 Antibody |
| Product Description: | Chicken Polyclonal anti-MAP 2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Cow brain MAP 2 purified from the material that pellets with GTP polymerized tubulin (see references). |
| Cross Reactivity: | Cross-reacts with Cow, Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.25 ml Affinity purified Chicken antisera. |
| Uses: | IF: Use at a dilution of 1/30000. WB: Use at a dilution of 1/300000. Detects a band of approximately 280 kDa, corresponding to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
| Localization: | Cytoplasmic |
| Background: | MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Bv, Hu, Mu, Rt, |
| Alternate Names: | anti-Dendrite specific MAP antibody, anti-DKFZp686I2148 antibody, anti-MAP 2 antibody, anti-MAP2 antibody, anti-MAP2A antibody, anti-MAP2B antibody, anti-MAP2C antibody, anti-Microtubule associated protein 2 antibody, anti-Mtap 2 antibody |
| Package Size: | 0.25 ml |
| Preservative: | Sodium Azide |
| General References: | Vallee RB Purification of brain microtubules and microtubule-associated protein 1 using taxol. Methods Enzymol 134:104-15 (1986). Vallee RB Reversible assembly purification of microtubules without assembly-promoting agents and further purification of tubulin, microtubule-associated proteins, and MAP fragments. Methods Enzymol 134:89-104 (1986). |
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