| CatalogCode: | NB120-5203 |
| ProductName: | Hck Antibody |
| Product Description: | Rabbit Polyclonal anti-Hck [Tyr207/Ser211] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of mouse Hck that contains tyrosine 207 and serine 209 (tyrosine 209 and serine 211 in human). |
| Specificity: | There is some cross-reactivity with Src family kinases Lck and Lyn, but the antibody does not react with Src, Fyn, or Yes. |
| CrossReactivity: | Cross-reacts with Rat.Expected to cross-react with Human (92% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.35 - 1.0 ug/ml. Predicted molecular weight: 63 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic. |
| Control: | PC12 cells +/- sorbitol, Chick Embryo Fibroblast (CEF) cells expressing Src protein and plated on fi |
| Background: | Hck is a member of the Src family of non-receptor tyrosine kinases involved in the modulation of a wide variety of normal and pathogenic cellular processes including growth, development, differentiation, and carcinogenesis. These proteins contain a consensus structure comprised of one or more N-terminal SH2 and/or SH3 domains involved in protein-protein interactions, a central catalytic tyrosine kinase domain, and a short C terminal domain. This C-terminal domain contains a tyrosine residue (tyrosine 522) that, when phosphorylated by Csk tyrosine kinase, functions as a negative regulatory site by providing a binding site for the N-terminal SH2 domain. The association of these two domains blocks access to a critical tyrosine residue in the activation loop, the phosphorylation of which is required for enzyme activation. Release of this autoinhibition can be achieved either by dephosphorylation of tyrosine 522 or by phosphorylation of tyrosine 209 and/or serine 211 in the SH2 domain. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Rt |
| ALTnames: | anti-Hck 1 antibody, anti-Hemopoietic Cell Kinase antibody, anti-Hemopoietic cell kinase isoform p59 Hck antibody, anti-JTK 9 antibody, anti-JTK9 antibody, anti-MGC 18625 antibody, anti-p59 HCK/p60 HCK antibody, anti-p59HCK/p60HCK antibody, anti-Tyrosine protein kinase HCK antibody |
| ProteinTarget: | Hck |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Young MA et al. Dynamic coupling between the SH2 and SH3 domains of c-Src and Hck underlies their inactivation by C-terminal tyrosine phosphorylation. Cell 105:115-26 (2001). Hubbard SR & Till JH Protein tyrosine kinase structure and function. Annu Rev Biochem 69:373-98 (2000). Thomas SM & Brugge JS Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13:513-609 (1997). Stover DR et al. Modulation of the SH2 binding specificity and kinase activity of Src by tyrosine phosphorylation within its SH2 domain. J Biol Chem 271:12481-7 (1996). |
| Phosphorylated: | Tyr207/Ser211 |