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| Catalog Code: | NB120-5191 |
| Product Name: | Integrin beta 3 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Integrin beta 3 [Tyr785] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the c-terminus of the Integrin beta 3 receptor (based on Swiss Protein database, accession number P05106). The sequence is conserved in human, mouse and rat. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Chicken (85% identity with immunogen), Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 130 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Background: | Human Integrin beta 3 (CD61) antigen is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. The integrin beta 3 chain of the vitronectin receptor and GPIIb/IIIa complex. The beta 3 integrin chain is a 90-110 kDa glycoprotein polypeptide which is expressed on platelets, megakaryocytes, macrophages, osteoclasts and synovial lining cells. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-CD 61 antibody, anti-CD61 antibody, anti-CD61 antigen antibody, anti-GP3A antibody, anti-GPIIIa antibody, anti-HPA 1 antibody, anti-HPA 4 antibody, anti-Integrin beta 3 chain antibody, anti-ITG B3 antibody, anti-ITGB 3 antibody, anti-ITGB3 antibody, anti-NAIT antibody, anti-Platelet Fibrinogen Receptor Beta Subunit antibody, anti-Platelet Glycoprotein IIIa antibody, anti-platelet glycoprotein IIIa precursor antibody, anti-Platelet membrane glycoprotein IIIa antibody, anti-PTP antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Tyr785 |
| General References: | General / background references:Maile LA et al. Structural analysis of the role of the beta 3 subunit of the alpha V beta 3 integrin in IGF-I signaling. J Cell Sci 114:1417-25 (2001). Boettiger D et al. Distinct ligand-binding modes for integrin alpha(v)beta(3)-mediated adhesion to fibronectin versus vitronectin. J Biol Chem 276:31684-90 (2001). Cukierman E et al. Taking cell-matrix adhesions to the third dimension. Science 294:1708-12 (2001). Sanjay A et al. Cbl associates with Pyk2 and Src to regulate Src kinase activity, alpha(v)beta(3) integrin-mediated signaling, cell adhesion, and osteoclast motility. J Cell Biol 152:181-95 (2001). |
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