| CatalogCode: | NB120-5189 |
| ProductName: | Integrin beta 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Integrin beta 1 [Thr788/Thr789] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558). The sequence is conserved in human, mouse, rat and chicken. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Chicken,Mouse,Rat due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 130 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type 1 membrane protein |
| Control: | HeLa cells in mitosis. |
| Background: | Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-beta 1 subunit antibody, anti-CD29 antigen antibody, anti-Fibrinogen Receptor beta subunit antibody, anti-GPIIA antibody, anti-ITGB1 antibody, anti-MDF2 antibody, anti-Very Late Activation Protein beta Polypeptide antibody, anti-VLAB antibody, anti-VLAbetaantibody |
| ProteinTarget: | Integrin beta 1 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Blystone SD et al. A molecular mechanism of integrin crosstalk: alphavbeta3 suppression of calcium/calmodulin-dependent protein kinase II regulates alpha5beta1 function. J Cell Biol 145:889-97 (1999). Schlaepfer DD et al. Signaling through focal adhesion kinase. Prog Biophys Mol Biol 71:435-78 (1999). Wennerberg K et al. Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin beta1A. Requirement for threonines 788-789 in receptor activation. J Cell Sci 111 ( Pt 8):1117-26 (1998). Richardson A & Parsons JT Signal transduction through integrins: a central role for focal adhesion kinase? Bioessays 17:229-36 (1995). |
| Phosphorylated: | Thr788/Thr789 |
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