| CatalogCode: | NB120-5184 |
| ProductName: | Integrin alpha 4 Antibody |
| Product Description: | Rabbit Polyclonal anti-Integrin alpha 4 [Ser988] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human integrin alpha 4 that contains serine 988. |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.05 ml Whole Rabbit antisera. |
| Uses: | IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 150 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Control: | Jurkat T cells transfected with full-length, wild-type or S988A mutant integrin alpha 4. |
| Background: | ntegrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery. |
| Storage: | Store at −80oC. Upon initial thawing, apportion into working aliquots and store at −80oC. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 285 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Alpha 4 subunit of VLA 4 receptor antibody, anti-Antigen CD49D antibody, anti-CD 49d antibody, anti-CD49d antibody, anti-CD49d antigen antibody, anti-CDw49d antibody, anti-IA4 antibody, anti-Integrin alpha IV antibody, anti-ITGA 4 antibody, anti-ITGA4 antibody, anti-LPAM23 antibody, anti-MGC90518 antibody, anti-Very Late Activation Antigen 4 antibody, anti-Very Late Activation Protein 4 Receptor Alpha 4 Subunit antibody, anti-VLA 4 antibody, anti-VLA4 antibody |
| ProteinTarget: | Integrin alpha 4 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Liu S et al. A fragment of paxillin binds the alpha 4 integrin cytoplasmic domain (tail) and selectively inhibits alpha 4-mediated cell migration. J Biol Chem 277:20887-94 (2002). Jackson DY Alpha 4 integrin antagonists. Curr Pharm Des 8:1229-53 (2002). Han J et al. Phosphorylation of the integrin alpha 4 cytoplasmic domain regulates paxillin binding. J Biol Chem 276:40903-9 (2001). Liu S & Ginsberg MH Paxillin binding to a conserved sequence motif in the alpha 4 integrin cytoplasmic domain. J Biol Chem 275:22736-42 (2000). Arroyo AG et al. In vivo roles of integrins during leukocyte development and traffic: insights from the analysis of mice chimeric for alpha 5, alpha v, and alpha 4 integrins. J Immunol 165:4667-75 (2000). |
| Phosphorylated: | Ser988 |
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