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| Catalog Code: | NB120-4901 |
| Product Name: | Lck Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Lck [Tyr505] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from the region of Lck that contains tyrosine 505 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse. |
| Specificity: | Hck [pY522] (75% homology), and Lyn [pY508] (83.3% homology) have not been tested. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Chicken (92% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 60 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilise Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-Lck p56 antibody, anti-Lymphocyte Specific Protein Tyrosine Kinase antibody, anti-Membrane associated protein tyrosine kinase antibody, anti-Oncogene lck antibody, anti-P56 LCK antibody, anti-Proto oncogene tyrosine protein kinase LCK antibody, anti-T cell specific protein tyrosine kinase antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Tyr505 |
| General References: | General / background references:Fujimaki W et al. Functional uncoupling of T-cell receptor engagement and Lck activation in anergic human thymic CD4+ T cells. J Biol Chem 276:17455-60 (2001). Ellery JM et al. Activation of the interleukin 2 receptor: a possible role for tyrosine phosphatases. Cell Signal 12:367-73 (2000). Gervais FG & Veillette A Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J Biol Chem 272:12754-61 (1997). Hardwick JS & Sefton BM The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505. J Biol Chem 272:25429-32 (1997). |
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