| CatalogCode: | | NB120-4900 |
| ProductName: | | Lck Antibody |
| Product Description: | | Rabbit Polyclonal anti-Lck [Ser192] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (Human) derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse. |
| Specificity: | | Lck (79% homologous), Hck (79% homologous) and Lyn (93% homologous) have not been tested. |
| CrossReactivity: | | Cross-reacts with Human.Expected to cross-react with Chicken (79% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 60 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic |
| Control: | | Full length untagged recombinant human Lck protein. |
| Background: | | Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of multiple sites, including the two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Phosphorylation of Lck Tyr-192 blocks high affinity binding of other proteins to the SH2 domain of Lck, thereby negatively regulating its participation in T cell antigen receptor signalling. |
| Storage: | | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Lck p56 antibody, anti-Lymphocyte Specific Protein Tyrosine Kinase antibody, anti-Membrane associated protein tyrosine kinase antibody, anti-Oncogene lck antibody, anti-P56 LCK antibody, anti-Proto oncogene tyrosine protein kinase LCK antibody, anti-T cell specific protein tyrosine kinase antibody |
| ProteinTarget: | | Lck |
| PackageSize: | | 0.05 ml |
| GeneralRef: | | General / background references:Fujimaki W et al. Functional uncoupling of T-cell receptor engagement and Lck activation in anergic human thymic CD4+ T cells. J Biol Chem 276:17455-60 (2001). Ellery JM et al. Activation of the interleukin 2 receptor: a possible role for tyrosine phosphatases. Cell Signal 12:367-73 (2000). Gervais FG & Veillette A Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J Biol Chem 272:12754-61 (1997). Hardwick JS & Sefton BM The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505. J Biol Chem 272:25429-32 (1997). |
| Phosphorylated: | | Ser192 |
| more information: | | company product webpage |