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| Catalog Code: | NB120-4833 |
| Product Name: | Paxillin Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Paxillin [Tyr118] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 118. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Expected to cross-react with Chicken (87% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.5 - 1.0 ug/ml. Predicted molecular weight: 68 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Storage: | Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk). |
| Purity: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Isotype: | Immunogen affinity purified |
| Host Name: | IgG |
| Buffer: | Rabbit |
| Application Summary: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Species Summary: | Western Blot |
| Alternate Names: | Hu, Mu, |
| Package Size: | anti-FLJ16691 antibody, anti-FLJ23042 antibody, anti-Paired box protein Pax 1 antibody, anti-PAX 1 antibody, anti-PAX1 antibody, anti-Paxillin alpha antibody, anti-PXN antibody, anti-PXN protein antibody |
| Preservative: | 0.05 ml |
| Homology: | 0.05% sodium azide |
| Notes Main: | Tyr118 |
| Product References: | This product has been used in:Manabe Ri R et al. GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J Cell Sci 115:1497-510 (2002). Nikolopoulos SN & Turner CE Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem 277:1568-75 (2002). van de Water B et al. Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury. J Biol Chem 276:36183-93 (2001). Weinberg JM et al. Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am J Pathol 158:2153-64 (2001). West KA et al. The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL). J Cell Biol 154:161-76 (2001). Schaller MD & Schaefer EM Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin. Biochem J 360:57-66 (2001). Nakamura K et al. Tyrosine phosphorylation of paxillin alpha is involved in temporospatial regulation of paxillin-containing focal adhesion formation and F-actin organization in motile cells. J Biol Chem 275:27155-64 (2000). |
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