| CatalogCode: | | NB120-4819 |
| ProductName: | | ERK 1 + 2 Antibody |
| Product Description: | | Rabbit Polyclonal anti-ERK 1 + 2 [Thr185/Thr202] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (Human) derived from the region of ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187, based on the human sequence. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly. |
| CrossReactivity: | | Cross-reacts with Human.Expected to cross-react with a wide range of other species due to sequence homology.Not yet tested in other species. |
| Packaging: | | 0.5 ml |
| Uses: | | WB: Use at a dilution of 1/1000. Predicted molecular weight: 44,42 kDa. Can be blocked with <ab5354>NIH3T3 whole cell lysate, + / - PDGF stimulation (pair)</ab>, <ab5313>PC12 whole cell lysate, + / - Sorbitol treatment</ab> or <ab5255>ERK 1 + 2 phospho T185 / 202 + Y187 / 204 and non-phospho peptides (pair)</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | | PC12 cells +/- Sorbitol. |
| Background: | | ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signalling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK 1 and ERK 2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK 1 + 2 enzymes. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH |
| ListPrice: | | 330 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ProteinTarget: | | ERK 1 + 2 |
| PackageSize: | | 0.05 ml |
| GeneralRef: | | This product has been used in:Rapoport M et al. Tau is essential to beta -amyloid-induced neurotoxicity. Proc Natl Acad Sci U S A 99:6364-9 (2002). Robinson FL et al. Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1. J Biol Chem 277:14844-52 (2002). Grammatikakis N et al. The role of Hsp90N, a new member of the Hsp90 family, in signal transduction and neoplastic transformation. J Biol Chem 277:8312-20 (2002). Tong T et al. Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation. Exp Cell Res 269:64-72 (2001). Janulis M et al. A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity. Mol Cell Biol 21:2235-47 (2001). Nanki T et al. Chemokines regulate IL-6 and IL-8 production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. J Immunol 167:5381-5 (2001). |
| Phosphorylated: | | Thr185/Thr202 |
| more information: | | company product webpage |
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