| CatalogCode: | NB120-4817 |
| ProductName: | Src Antibody |
| Product Description: | Rabbit Polyclonal anti-Src [Tyr529] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, based on the human sequence. The sequence is conserved in mouse (tyrosine 534), chicken (tyrosine 527) and frog (tyrosine 525). |
| Specificity: | Fyn and Yes (92%homologous) were not tested. |
| CrossReactivity: | Cross-reacts with Human, Chicken, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Predicted molecular weight: 62 kDa. Can be blocked with <ab5257>Src phospho Y529 and non-phospho peptides (pair)</ab> or <ab5353>CEF whole cell lysate, positive / negative for Src (pair)</ab>Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin. |
| Background: | Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylatedleading to full activation. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Ch, Hu, Mu, Rt |
| ALTnames: | anti-ASV antibody, anti-cSrc antibody, anti-Neuronal CSRC tyrosine specific protein kinase antibody, anti-p60Src antibody, anti-pp60csrc antibody, anti-protooncogene SRC Rous sarcoma antibody, anti-Tyrosine kinase pp60c src antibody, anti-Tyrosine protein kinase SRC1 antibody, anti-vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody |
| ProteinTarget: | Src |
| PackageSize: | 0.05 ml |
| GeneralRef: | This product has been used in:Haas M et al. Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J Biol Chem 277:18694-702 (2002). Suhr SM et al. Antisense oligodeoxynucleotide evidence that a unique osteoclastic protein-tyrosine phosphatase is essential for osteoclastic resorption. J Bone Miner Res 16:1795-803 (2001). Gil-Henn H et al. Regulation of protein-tyrosine phosphatases alpha and epsilon by calpain-mediated proteolytic cleavage. J Biol Chem 276:31772-9 (2001). Cheng A et al. Attenuation of adhesion-dependent signaling and cell spreading in transformed fibroblasts lacking protein tyrosine phosphatase-1B. J Biol Chem 276:25848-55 (2001). Giusti AF et al. Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase cgamma. J Biol Chem 275:16788-94 (2000). Ma YC et al. Src tyrosine kinase is a novel direct effector of G proteins. Cell 102:635-46 (2000). Sussman MA et al. Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1. J Clin Invest 105:875-86 (2000). Maher PA Disruption of cell-substrate adhesion activates the protein tyrosine kinase pp60(c-src). Exp Cell Res 260:189-98 (2000). Osusky M et al. Autophosphorylation of purified c-Src at its primary negative regulation site. J Biol Chem 270:25729-32 (1995). |
| Phosphorylated: | Tyr529 |