| CatalogCode: | NB120-4805 |
| ProductName: | PYK 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-PYK 2 [Tyr579] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 579. The sequence is conserved in mouse and rat. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.2 - 1.0 ug/ml. Predicted molecular weight: 116 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | Chicken embryo fibroblasts expressing human PYK 2 protein and plated on fibronectin. |
| Background: | Proline-rich/Ca2+ -activated tyrosine kinase (PYK 2) is a member of the Focal Adhesion Kinase family of non-receptor, proline-rich PTK's. PYK 2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines, and certain stress stimuli. PYK 2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death. Phosphorylation of the catalytic domain tyrosines 579 and 580 by Src results in maximum activation of PYK 2. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-FADK antibody, anti-FAK 2 antibody, anti-FAK antibody, anti-FAK1 antibody, anti-Focal Adhesion Kinase 2 antibody, anti-pp125FAK antibody, anti-Proline Rich Tyrosine Kinase 2 antibody, anti-Protein Tyrosine Kinase 2 Beta antibody, anti-PTK 2B antibody |
| ProteinTarget: | PYK 2 |
| PackageSize: | 0.05 ml |
| GeneralRef: | This product has been used in:McCole DF et al. Transactivation of the epidermal growth factor receptor in colonic epithelial cells by carbachol requires extracellular release of transforming growth factor-alpha. J Biol Chem 277:42603-12 (2002). Nakamura K et al. Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20:2626-35 (2001). Sorokin A et al. Protein-tyrosine kinase Pyk2 mediates endothelin-induced p38 MAPK activation in glomerular mesangial cells. J Biol Chem 276:21521-8 (2001). Klingbeil CK et al. Targeting Pyk2 to beta 1-integrin-containing focal contacts rescues fibronectin-stimulated signaling and haptotactic motility defects of focal adhesion kinase-null cells. J Cell Biol 152:97-110 (2001). Lyons PD et al. Inhibition of the catalytic activity of cell adhesion kinase beta by protein-tyrosine phosphatase-PEST-mediated dephosphorylation. J Biol Chem 276:24422-31 (2001). Katagiri T et al. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem 275:19645-52 (2000). Keely SJ et al. Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular ca(2+), PYK-2, and p60(src). J Biol Chem 275:12619-25 (2000). Sato T Enhancement of gustatory neural response to salts following adaptation of frog tongue to quinine-HCI. Tohoku J Exp Med 117:381-4 (1975). |
| Phosphorylated: | Tyr579 |
order or
more information: | company product webpage |
| request information: |
|