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| Catalog Code: | NB120-4801 |
| Product Name: | PYK 2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PYK 2 [Tyr881] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 881. |
| Specificity: | Some cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system. |
| Cross Reactivity: | Cross-reacts with Chicken and Human.Expected to cross-react with Mouse (86% identity with immunogen), Rat (86% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC: Use at an assay dependant dilution. WB: Use at a concentration of 0.5 - 1.0 ug/ml. Predicted molecular weight: 116 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Proline-rich/Ca2+ -activated tyrosine kinase (PYK 2) is a member of the Focal Adhesion Kinase family of non-receptor, proline-rich PTK's. PYK 2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines, and certain stress stimuli. PYK 2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death. pTyr881 is the potential binding site of Grb 2. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | immunohistochemistry, Western Blot |
| Species Summary: | Ch, Hu, |
| Alternate Names: | anti-FADK antibody, anti-FAK 2 antibody, anti-FAK antibody, anti-FAK1 antibody, anti-Focal Adhesion Kinase 2 antibody, anti-pp125FAK antibody, anti-Proline Rich Tyrosine Kinase 2 antibody, anti-Protein Tyrosine Kinase 2 Beta antibody, anti-PTK 2B antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Tyr881 |
| General References: | This product has been used in:Sajan MP et al. Sorbitol activates atypical protein kinase C and GLUT4 glucose transporter translocation/glucose transport through proline-rich tyrosine kinase-2, the extracellular signal-regulated kinase pathway and phospholipase D. Biochem J 362:665-74 (2002). Sorokin A et al. Protein-tyrosine kinase Pyk2 mediates endothelin-induced p38 MAPK activation in glomerular mesangial cells. J Biol Chem 276:21521-8 (2001). Bandyopadhyay G et al. Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation. J Biol Chem 276:35537-45 (2001). Nakamura K et al. Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20:2626-35 (2001). Katagiri T et al. Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem 275:19645-52 (2000). Shi CS et al. G13alpha-mediated PYK2 activation. PYK2 is a mediator of G13alpha -induced serum response element-dependent transcription. J Biol Chem 275:24470-6 (2000). Keely SJ et al. Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular ca(2+), PYK-2, and p60(src). J Biol Chem 275:12619-25 (2000). |
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