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| Catalog Code: | NB120-4794 |
| Product Name: | Focal Adhesion Kinase Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Focal Adhesion Kinase [Ser910] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog. |
| Specificity: | Phosphorylation site-specific antibody (PSSA) selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on serine 910. The antibody has been shown to recognize FAK (approximately 125 kDa) in human epithelial carcinoma cells. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Chicken, Mouse, Rat, Xenopus laevis due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 125 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic, localized to focal adhesions. |
| Background: | FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-FADK 1 antibody, anti-FADK antibody, anti-FAK 1 antibody, anti-FAK1 antibody, anti-Focal adhesion kinase 1 antibody, anti-pp125FAK antibody, anti-Protein tyrosine kinase 2 antibody, anti-Protein Tyrosine Kinase Cytoplasmic antibody, anti-PTK 2 antibody, anti-PTK2 antibody, anti-PTK2 protein tyrosine kinase 2 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Ser910 |
| General References: | This product has been used in:Ma A et al. Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130(Cas). Mol Biol Cell 12:1-12 (2001). General / background references:Xu LH et al. The focal adhesion kinase suppresses transformation-associated, anchorage-independent apoptosis in human breast cancer cells. Involvement of death receptor-related signaling pathways. J Biol Chem 275:30597-604 (2000). Yamakita Y et al. Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK. J Cell Biol 144:315-24 (1999). Richardson A et al. Identification of integrin-stimulated sites of serine phosphorylation in FRNK, the separately expressed C-terminal domain of focal adhesion kinase: a potential role for protein kinase A. Biochem J 324 ( Pt 1):141-9 (1997). |
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more information: | company product webpage |