| CatalogCode: | NB120-4790 |
| ProductName: | Focal Adhesion Kinase Antibody |
| Product Description: | Rabbit Polyclonal anti-Focal Adhesion Kinase [Ser722] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide phosphopeptide derived from the region of FAK that contains serine 722. |
| Specificity: | Phosphorylation site-specific antibody (PSSA) selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on serine 722. The antibody has been shown to recognize FAK (approximately 125 kDa) in human epithelial carcinoma cells. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Chicken, Mouse, Rat, Xenopus laevis due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 125 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic, localized to focal adhesions. |
| Background: | Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Focal Adhesion Kinase regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. The role of phosphorylation at Ser722 is currently being actively investigated. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-FADK 1 antibody, anti-FADK antibody, anti-FAK 1 antibody, anti-FAK1 antibody, anti-Focal adhesion kinase 1 antibody, anti-pp125FAK antibody, anti-Protein tyrosine kinase 2 antibody, anti-Protein Tyrosine Kinase Cytoplasmic antibody, anti-PTK 2 antibody, anti-PTK2 antibody, anti-PTK2 protein tyrosine kinase 2 antibody |
| ProteinTarget: | Focal Adhesion Kinase phospho |
| PackageSize: | 0.05 ml |
| GeneralRef: | This product has been used in:Ma A et al. Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130(Cas). Mol Biol Cell 12:1-12 (2001). General / background references:Ridyard MS & Sanders EJ Potential roles for focal adhesion kinase in development. Anat Embryol (Berl) 199:1-7 (1999). Yamakita Y et al. Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK. J Cell Biol 144:315-24 (1999). Richardson A et al. Identification of integrin-stimulated sites of serine phosphorylation in FRNK, the separately expressed C-terminal domain of focal adhesion kinase: a potential role for protein kinase A. Biochem J 324 ( Pt 1):141-9 (1997). |
| Phosphorylated: | Ser722 |