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| Catalog Code: | NB120-4779 |
| Product Name: | Rb Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Rb [Thr826] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from a region of human Rb that contains threonine 826 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse, rat, and chicken. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Chicken (100% identity with immunogen), Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.25 - 1.0 ug/ml. Predicted molecular weight: 106 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-OSRC antibody, anti-P105RB antibody, anti-PP105 antibody, anti-PP110 antibody, anti-pRb antibody, anti-Retinoblastoma 1 antibody, anti-Retinoblastoma antibody, anti-Retinoblastoma associated protein antibody, anti-Retinoblastoma related osteosarcoma antibody, anti-Retinoblastoma susceptibility gene antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr826 |
| General References: | General / background references:Brantley MA & Harbour JW The molecular biology of retinoblastoma. Ocul Immunol Inflamm 9:1-8 (2001). Rubin E et al. Site-specific and temporally-regulated retinoblastoma protein dephosphorylation by protein phosphatase type 1. Oncogene 20:3776-85 (2001). Tamrakar S et al. Role of pRB dephosphorylation in cell cycle regulation. Front Biosci 5:D121-37 (2000). Brown VD et al. Cumulative effect of phosphorylation of pRB on regulation of E2F activity. Mol Cell Biol 19:3246-56 (1999). |
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more information: | company product webpage |