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| Catalog Code: | NB120-4768 |
| Product Name: | c-Raf Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-c-Raf [Ser338/Tyr340] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 338 and tyrosine 340. The sequence is conserved in mouse and rat. |
| Specificity: | A-Raf (57%) has not been tested, but is not expected to react. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.5 - 1.5 ug/ml. Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Mitochondrial outer membrane |
| Background: | The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Phosphorylation of serine 338 creates a recognition site for tyrosine kinases and is critical for the activation of c-Raf. Serine 338 to tyrosine 341 (SSYY) is the phosphoregulatory site which regulates the biological function of the c-Raf tyrosine kinase by oncogenic Ras and Src. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-Craf 1 transforming gene antibody, anti-cRaf antibody, anti-Craf1 transforming gene antibody, anti-Murine sarcoma 3611 oncogene 1 antibody, anti-RAF antibody, anti-Raf proto oncogene serine/threonine protein kinase antibody, anti-vraf1 murine leukemia viral oncogene homolog 1 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Ser338/Tyr340 |
| General References: | General / background references:Carey KD et al. The requirement of specific membrane domains for Raf-1 phosphorylation and activation. J Biol Chem 278:3185-96 (2003). Zhang BH & Guan KL Regulation of the Raf kinase by phosphorylation. Exp Lung Res 27:269-95 (2001). Mason CS et al. Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation. EMBO J 18:2137-48 (1999). Diaz B et al. Phosphorylation of Raf-1 serine 338-serine 339 is an essential regulatory event for Ras-dependent activation and biological signaling. Mol Cell Biol 17:4509-16 (1997). |
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