| Catalog Code: | NB120-4766 |
| Product Type: | Primary Antibody |
| Product Name: | c-Raf Antibody |
| List Price: | 330 |
| Clonality: | Polyclonal |
| Gene ID: | 5894 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-c-Raf [Ser259] |
| Phosphorylated: | Ser259 |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Species Summary: | Hu |
| Background: | The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription fac |
| Alternate Names: | anti-Craf 1 transforming gene antibody, anti-cRaf antibody, anti-Craf1 transforming gene antibody, anti-Murine sarcoma 3611 oncogene 1 antibody, anti-RAF antibody, anti-Raf proto oncogene serine/threonine protein kinase antibody, anti-vraf1 murine leukemi |
| Immunogen: | Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 259. The sequence is conserved in mouse and rat. |
| Specificity: | A-Raf (90%) was also shown to react in HCT-8 cells, a human cancer cell line expressing high levels of A-Raf. |
| Purity: | Immunogen affinity purified |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
| Packaging: | 50 microlitres |
| Package Size: | 0.05 ml |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Application Summary: | WB |
| General References: | General / background references:Dhillon AS et al. Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. Mol Cell Biol 22:3237-46 (2002). Dumaz N et al. Cyclic AMP blocks cell growth through Raf-1-depe |