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| Catalog Code: | NB120-4756 |
| Product Name: | MEK2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-MEK2 [Thr394] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody is not expected to react with MEK 1, which is 64% homologous. |
| Cross Reactivity: | Cross-reacts with Human.Does not cross-react with Mouse or Rat.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Predicted molecular weight: 48 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), Mu (-), Rt (-), |
| Alternate Names: | anti-Cardiofaciocutaneous syndrome antibody, anti-CFC syndrome antibody, anti-Dual specificity mitogen activated protein kinase kinase 2 antibody, anti-ERK activator kinase 2 antibody, anti-MAP kinase kinase 2 antibody, anti-MAP2K 2 antibody, anti-MAPK / ERK kinase 2 antibody, anti-MAPKK 2 antibody, anti-MAPKK2 antibody, anti-MEK 2 antibody, anti-Microtubule Associated Protein Kinase Kinase 2 antibody, anti-Mitogen activated protein kinase kinase 2 p45 antibody, anti-MKK 2 antibody, anti-MKK2 antibody, anti-PRKMK 2 antibody, anti-PRKMK2 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr394 |
| General References: | General / background references:Jin K et al. MEK and ERK protect hypoxic cortical neurons via phosphorylation of Bad. J Neurochem 80:119-25 (2002). Lin CC et al. Thrombin-stimulated cell proliferation mediated through activation of Ras/Raf/MEK/MAPK pathway in canine cultured tracheal smooth muscle cells. Cell Signal 14:265-75 (2002). Sharma P et al. Phosphorylation of MEK1 by cdk5/p35 down-regulates the mitogen-activated protein kinase pathway. J Biol Chem 277:528-34 (2002). Takahashi H et al. Expression of human cystatin A by keratinocytes is positively regulated via the Ras/MEKK1/MKK7/JNK signal transduction pathway but negatively regulated via the Ras/Raf-1/MEK1/ERK pathway. J Biol Chem 276:36632-8 (2001). |
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more information: | company product webpage |