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| Catalog Code: | NB120-4742 |
| Product Name: | STAT1 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-STAT1 [Ser277] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) derived from the region of human STAT 1 that contains serine 727. This region is conserved between human and mouse. |
| Specificity: | This antibody does not react with non-phosphorylated STAT 1 protein. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.2 - 1.0 ug/ml. Predicted molecular weight: 91 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the STAT proteins are poorly defined at this time. They are thought to be involved in a variety of systems including antiviral responses and cell transformation. STAT1 deficient knock-out mice are unable to induce IFN regulated genes and are extremely susceptible to viral diseases. After phosphorylation, STAT1 and STAT2 form heterodimers that function as more potent inducers of transcription than the STAT1 homodimer. STAT1 is known to associate with the transcription factors p48, Sp1, and p300. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. STAT1 alpha and STAT1 beta isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1 beta lacks 38 carboxy terminal amino acids. Contained within these 38 terminal amino acids of STAT1 alpha is a critical serine residue (Ser 727) whose phosphorylation is required for maximal transcription activity induced by IFN gamma. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-DKFZp686B04100 antibody, anti-ISGF 3 antibody, anti-Signal transducer and activator of transcription 1 91kDa antibody, anti-Signal transducer and activator of transcription 1 alpha/beta antibody, anti-Signal Transductor and Activator of Transcription 1 antibody, anti-STAT 1 antibody, anti-STAT 91 antibody, anti-STAT91 antibody, anti-Transcription factor ISGF 3 components p91 p84 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Ser277 |
| General References: | This product has been used in:Burysek L et al. The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways. J Biol Chem 277:33509-17 (2002). General / background references:Ihle JN STATs: signal transducers and activators of transcription. Cell 84:331-4 (1996). Wen Z et al. Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Cell 82:241-50 (1995). Schindler C & Darnell JE Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 64:621-51 (1995). |
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more information: | company product webpage |