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| Catalog Code: | NB120-4721 |
| Product Name: | DOK 2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-DOK 2 [Tyr142] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Mouse)derived from a region of mouse Dok-2 that contains tyrosine 142 (tyrosine 139 in the human sequence). |
| Specificity: | This antibody should not react with Dok 1, which is 50% homologous to Dok 2. |
| Cross Reactivity: | Cross-reacts with Mouse.Expected to cross-react with Human (80% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Detects a band of approximately 56 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Dok 2/Dok R (downstream of tyrosine kinases, aka p56dok) is a 56 kDa protein and a member of the Dok family of RasGAP binding proteins. Dok-2 plays an important role downstream of several growth factor receptors, including VEGFR and EGFR, and is implicated in negative regulation of signaling pathways activated by tyrosine kinases. Dok-2 associates with Tek following angiopoietin-1 stimulation, resulting in tyrosine phosphorylation of Dok 2 and the subsequent recruitment of Nck and the p21 activating kinase (PAK/PAK1) to the activated receptor. Dok 2 can also be tyrosine phosphorylated by Lyn, ErbB-2/Neu, Bcr/Abl and rasGAP. Dok 2 tyrosine 142 is conserved with Dok 1 tyrosine 146, a phosphorylation site that inhibits Src transformation. |
| Storage: | Store at −80oC. Upon initial thawing, apportion into working aliquots and store at −80oC. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), with 1.0 mg/mL BSA (IgG, protea |
| Application Summary: | Western Blot |
| Species Summary: | Mu, |
| Alternate Names: | anti-Docking protein 2 56kDa antibody, anti-Docking protein 2 antibody, anti-DOK 2 antibody, anti-DOK R antibody, anti-Downstream of tyrosine kinase 2 antibody, anti-p56(dok 2) antibody, anti-p56dok 2 antibody, anti-p56DOK antibody, anti-p56dok2 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% Sodium Azide |
| Phosphorylated: | Tyr142 |
| General References: | General / background references:Master Z et al. Dok-R plays a pivotal role in angiopoietin-1-dependent cell migration through recruitment and activation of Pak. EMBO J 20:5919-28 (2001). Dankort D et al. Multiple ErbB-2/Neu Phosphorylation Sites Mediate Transformation through Distinct Effector Proteins. J Biol Chem 276:38921-8 (2001). Lock P et al. Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck. J Biol Chem 274:22775-84 (1999). Jones N & Dumont DJ Recruitment of Dok-R to the EGF receptor through its PTB domain is required for attenuation of Erk MAP kinase activation. Curr Biol 9:1057-60 (1999). |
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