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| Catalog Code: | NB120-3684 |
| Product Name: | ICEBERG Antibody |
| Product Description: | Rabbit Polyclonal anti-ICEBERG |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody reacts predominantly with ICEBERG. |
| Cross Reactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Western Blot, ELISA Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of approximately 10 kDa rat brain lysate, which corresponds to the predicted molecular weight of ICEBERG.Samples were boiled prior to subjecting to a15% SDS-polyacrylamide gel electrophoresis. |
| Localization: | Cytoplasmic |
| Background: | A novel protein, ICEBERG, an inhibitor of pro-caspase 1 activation, has been studied. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The ICEBERG functions as a competitive antagonist of RIP 2/pro-caspase 1 interactions and inhibits caspase 1 dependent pro-L-1b secretion in mononuclear cells. ICEBERG is induced by proinflammatory stimuli, suggesting that it may be part of a negative feedback loop. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | ELISA 0.1-1.0 ìg/ml, Western Blot 0.5-2.0 ìg/ml |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-Caspase 1 inhibitor Iceberg antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.02% sodium azide |
| General References: | General / background references:Razmara M et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J Biol Chem 277:13952-8 (2002). Druilhe A et al. Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins. Cell Death Differ 8:649-57 (2001). Lee SH et al. Cop, a caspase recruitment domain-containing protein and inhibitor of caspase-1 activation processing. J Biol Chem 276:34495-500 (2001). Humke EW et al. ICEBERG: a novel inhibitor of interleukin-1beta generation. Cell 103:99-111 (2000). |
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