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| Catalog Code: | NB120-3671 |
| Product Name: | Glucocorticoid Receptor Antibody |
| Product Description: | Rabbit Polyclonal anti-Glucocorticoid Receptor |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: APIEKEIPKIHSDVSSEQQHLKGQTG, corresponding to amino acids 150-175 of Human Glucocorticoid Receptor. |
| Specificity: | This antibody detects both the unactivated and activated forms of GR. |
| Cross Reactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.2 ml Whole Rabbit antisera. |
| Uses: | Western blot: use at a dilution of 1:100. Immunocytochemistry: use at a dilution of 1:100. Immunoprecipitation: assay dependent.Gel shift: assay dependent. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract. The recombinant GR protein in ab3582, Glucocorticoid Receptor Kit, can be used as a positive control; as can ab7943, Rat liver tissue lysate. For Gel shift assays, ab3582, Glucocorticoid Receptor Kit, needs to be purchased. |
| Localization: | Cytoplasmic in the absence of ligand; nuclear after ligand-binding. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunoprecipitation , Western Blot , Immunocytochemistry , Gel Super Shift Assays |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-GCCR antibody, anti-GCR antibody, anti-Glucocorticoid receptor lymphocyte antibody, anti-GR antibody, anti-GRL antibody, anti-Grl1 NR3C1 antibody, anti-Nuclear receptor subfamily 3 group C member 1 antibody |
| Package Size: | 0.2 ml |
| Preservative: | 0.05% sodium azide |
| General References: | General / background references:Roux S et al. Mutation of isoleucine 747 by a threonine alters the ligand responsiveness of the human glucocorticoid receptor. Mol Endocrinol 10:1214-26 (1996). Schuetz JD et al. Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells. Mol Pharmacol 49:63-72 (1996). Srinivasan G et al. Heat shock protein is tightly associated with the recombinant human glucocorticoid receptor:glucocorticoid response element complex. Mol Endocrinol 8:189-96 (1994). Beck CA et al. The steroid antagonist RU486 exerts different effects on the glucocorticoid and progesterone receptors. Endocrinology 133:728-40 (1993). Ashraf J & Thompson EB Identification of the activation-labile gene: a single point mutation in the human glucocorticoid receptor presents as two distinct receptor phenotypes. Mol Endocrinol 7:631-42 (1993). |
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