| CatalogCode: | NB120-3655 |
| ProductName: | GRB 2 Antibody |
| Product Description: | Mouse Monoclonal anti-GRB 2 (2GB04) |
| Clone: | 2GB04 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | This antibody reacts with a 24 - 25 kDa protein. |
| CrossReactivity: | Cross-reacts with Human, Rat, Mouse, Cow and Xenopus laevis. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Mouse ascites. |
| Uses: | Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/75 in an ABC method.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Cytoplasmic (probable) |
| Control: | Suitable tissue control sections for Immunohistochemistry:Tonsil. |
| Background: | Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| ListPrice: | 325 |
| AppSummary: | IHC |
| SpeciesSummary: | Bv, Hu, Mu, Rt, Xp |
| ALTnames: | anti-Abundant SRC homology antibody; anti-Ash protein antibody; anti-Epidermal growth factor receptor binding protein antibody; anti-GRB2 adapter protein antibody; anti-Growth Factor Receptor Bound Protein 2 antibody; anti-Growth factor receptor bound protein 3 antibody; anti-HT027 antibody; anti-SEM5 antibody; anti-SH2/SH3 adapter GRB2 antibody |
| ProteinTarget: | GRB 2 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Chardin P et al. The Grb2 adaptor. FEBS Lett 369:47-51 (1995). Downward J The GRB2/Sem-5 adaptor protein. FEBS Lett 338:113-7 (1994). |
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