| CatalogCode: | NB120-3589 |
| ProductName: | Calpain 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Calpain 1 (9A4H8D3) |
| Clone: | 9A4H8D3 |
| Clonality: | Monoclonal |
| Immunogen: | Purified bovine skeletal muscle 80 kDa Mu Calpain subunit. |
| Epitope: | This antibody recognizes an epitope between amino acids 465-520 (domain III) of human Mu Calpain. |
| Specificity: | NB120-3589 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. |
| CrossReactivity: | Cross-reacts with Human, Rat, Cow and Pig |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | Western blot: use at a dilution of 1/2000.Immunocytochemistry: use at a dilution of 1/100.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an 80 kDa protein representing Mu Calpain from human platelets and erythrocytes.Immunocytochemical staining of Mu Calpain in LLC-PK1 cells results in diffuse cytoplasmic staining. |
| Localization: | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. |
| Background: | The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| AppSummary: | IF, WB, ICC, ICC |
| SpeciesSummary: | Bv, Ha, Hu, Mu, Po, Rt, Rb |
| ALTnames: | anti-Calcium activated neutral proteinase 1 antibody, anti-Calcium activated neutral proteinase antibody, anti-Calpain 1 (mu/I) large subunit antibody, anti-Calpain 1 catalytic subunit antibody, anti-Calpain 1 large subunit antibody, anti-Calpain Large Polypeptide L1 antibody, anti-Calpain mu type antibody, anti-Calpain1 antibody, anti-CANP 1 antibody, anti-CANP antibody, anti-CANPL 1 antibody, anti-CANPL1 antibody, anti-CAPN 1 antibody, anti-CAPN1 antibody, anti-Cell proliferation inducing protein 30 antibody, anti-Micromolar Calpain antibody, anti-Mu Calpain antibody, anti-muCANP antibody, anti-muCL antibody |
| ProteinTarget: | Calpain 1 |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Boehm ML et al. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci 76:2415-34 (1998). General / background references:Cong J et al. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem 268:25740-7 (1993). Lane RD et al. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res 203:5-16 (1992). |
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