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| Catalog Code: | NB120-3579 |
| Product Name: | Glucocorticoid Receptor Antibody |
| Product Description: | Rabbit Polyclonal anti-Glucocorticoid Receptor |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: CKPLILPDTKPKIKD, corresponding to amino acids 245-259 of Human Glucocorticoid Receptor. |
| Specificity: | NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western blot: use at a concentration of 5ug/ml.Immunohistochemistry: use at a concentration of 1ug/ml.Immunoprecipitation: use at a concentration of 5ug/ml.Gel shift: assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. Immunohistochemical staining of GR in rat hippocampus results in staining primarily in the nucleus. |
| Localization: | Cytoplasmic in the absence of ligand; nuclear after ligand-binding. |
| Background: | Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Reconstitute with PBS. |
| Application Summary: | chromatin immunoprecipitation , immunoprecipitation 5 µg/ml, Western Blot 5 µg/ml, Immunocytochemistry , Immunohistochemistry-Frozen 1 µg/ml, Gel Super Shift Assays |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-GCCR antibody, anti-GCR antibody, anti-Glucocorticoid receptor lymphocyte antibody, anti-GR antibody, anti-GRL antibody, anti-Grl1 NR3C1 antibody, anti-Nuclear receptor subfamily 3 group C member 1 antibody |
| Package Size: | 0.05 mg |
| General References: | This product has been used in:Cidlowski JA et al. Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors. Mol Endocrinol 4:1427-37 (1990). General / background references:Smith CL et al. Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure. J Biol Chem 272:14227-35 (1997). Bellingham DL et al. Ligand-dependent down-regulation of stably transfected human glucocorticoid receptors is associated with the loss of functional glucocorticoid responsiveness. Mol Endocrinol 6:2090-102 (1992). McGimsey WC et al. Immunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies. Endocrinology 129:3064-72 (1991). Burnstein KL et al. Autoregulation of glucocorticoid receptor gene expression. Steroids 56:52-8 (1991). Burnstein KL et al. Evaluation of the role of ligand and thermal activation of specific DNA binding by in vitro synthesized human glucocorticoid receptor. Mol Endocrinol 5:1013-22 (1991). Burnstein KL et al. Human glucocorticoid receptor cDNA contains sequences sufficient for receptor down-regulation. J Biol Chem 265:7284-91 (1990). |
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