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| Catalog Code: | NB120-3577 |
| Product Name: | Estrogen Receptor beta Antibody |
| Product Description: | Rabbit Polyclonal anti-Estrogen Receptor beta |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: AEPQKSPWCEARSLEH, corresponding to amino acids 55-70 of Rat Estrogen Receptor beta. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse and Primate. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Gel shift: use 1-5ul.Western blot: use at a concentration of 1-2ug/ml.Immunohistochemistry: use at a concentration of 1-2ug/ml. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate.Immunohistochemical staining of ER beta in rat cerebellum results in cytoplasmic staining in Purkinje neurons.This antibody sufficiently supershifts rat ER beta / ER response element (vitellogenin xenopus A2 gene). |
| Localization: | Nuclear |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| Application Summary: | immunohistochemistry -P, Western Blot , Gel Super Shift Assays |
| Species Summary: | Hu, Mu, Mk, Rt, |
| Alternate Names: | anti-ER betaantibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.05% sodium azide |
| General References: | This product has been used in:Rosenfeld CS et al. Cloning, sequencing, and localization of bovine estrogen receptor-beta within the ovarian follicle. Biol Reprod 60:691-7 (1999). Rosenfeld CS et al. Transcription and translation of estrogen receptor-beta in the male reproductive tract of estrogen receptor-alpha knock-out and wild-type mice. Endocrinology 139:2982-7 (1998). General / background references:Makela S et al. Differentiation between vasculoprotective and uterotrophic effects of ligands with different binding affinities to estrogen receptors alpha and beta. Proc Natl Acad Sci U S A 96:7077-82 (1999). Alves SE et al. Differential colocalization of estrogen receptor beta (ERbeta) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain: an immunocytochemical study. Proc Natl Acad Sci U S A 95:3281-6 (1998). Krege JH et al. Generation and reproductive phenotypes of mice lacking estrogen receptor beta. Proc Natl Acad Sci U S A 95:15677-82 (1998). Petersen DN et al. Identification of estrogen receptor beta2, a functional variant of estrogen receptor beta expressed in normal rat tissues. Endocrinology 139:1082-92 (1998). Li X et al. Distribution of estrogen receptor-beta-like immunoreactivity in rat forebrain. Neuroendocrinology 66:63-7 (1997). |
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