| request information: |
|
| Catalog Code: | NB120-3576 |
| Product Name: | Estrogen Receptor beta Antibody |
| Product Description: | Rabbit Polyclonal anti-Estrogen Receptor beta |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: CSSTEDSKNKESSQNLQSQ, corresponding to amino acids 467-485 of Rat Estrogen Receptor beta. |
| Specificity: | This antibody detects estrogen receptor (ER) beta from human mouse and rat tissues. |
| Cross Reactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western blot: use at a concentration of 1ug/ml.Immunocytochemistry: use at a concentration of 1ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunocytochemical staining of ER beta in cells overexpressing the human protein results in nuclear staining. |
| Localization: | Nuclear |
| Background: | The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry 1:50, Western Blot 1 µg/ml, Immunocytochemistry 1 µg/ml, Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-ER BETA antibody, anti-Erb antibody, anti-ESR 2 antibody, anti-ESR B antibody, anti-ESR BETA antibody, anti-ESR2 antibody, anti-ESRB antibody, anti-ESTRB antibody, anti-Estrogen Receptor 2 antibody, anti-Estrogen receptor beta antibody, anti-NR3A2 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide, 1mg/ml BSA |
| General References: | General / background references:Schomberg DW et al. Targeted disruption of the estrogen receptor-alpha gene in female mice: characterization of ovarian responses and phenotype in the adult. Endocrinology 140:2733-44 (1999). Sar M & Welsch F Differential expression of estrogen receptor-beta and estrogen receptor-alpha in the rat ovary. Endocrinology 140:963-71 (1999). Azcoitia I et al. Localization of estrogen receptor beta-immunoreactivity in astrocytes of the adult rat brain. Glia 26:260-7 (1999). Krege JH et al. Generation and reproductive phenotypes of mice lacking estrogen receptor beta. Proc Natl Acad Sci U S A 95:15677-82 (1998). |
order or
more information: | company product webpage |