| CatalogCode: | NB120-3575 |
| ProductName: | Estrogen Receptor alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-Estrogen Receptor alpha |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: N(21)ELEPLNRPQLK(32)C.Immunizing peptide corresponds to amino acid residues 21-32 from human ER alpha. This peptide sequence is completely conserved between human, rat, mouse, rabbit, sheep, porcine and bovine species. |
| Specificity: | NB120-3575 detects estrogen receptor (ER) alpha from human, porcine and rat tissues and cells. |
| CrossReactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western blot: use at a concentration of 1ug/ml.Immunohistochemistry: use at a concentration of 1-5ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts.Immunohistochemical staining of ER alpha in rat prostate results in primarily nuclear staining. |
| Localization: | Nuclear |
| Background: | The Estrogen Receptor (ER) is a 67 kDa protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | IHC, IF, WB, IHC-P, IHC-Fr |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody |
| ProteinTarget: | Estrogen Receptor alpha |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Kuiper GG et al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology 138:863-70 (1997). Traish AM & Pavao M Binding of site-directed monoclonal antibodies to an epitope located in the A/B region (amino acids 140-154) of human estrogen receptor-induced conformational changes in an epitope in the DNA-binding domain. Steroids 61:549-56 (1996). |
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