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| Catalog Code: | NB120-3574 |
| Product Name: | Estrogen Receptor alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-Estrogen Receptor alpha |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: M(1)TMTLHTKASGMALLHQIQGN(21)C.Immunizing peptide corresponds to amino acid residues 1-21 of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken. |
| Cross Reactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Western blot: use at a concentration of 1ug/ml.Immunohistochemistry: use at a concentration of 1-5ug/ml. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts.Immunohistochemical staining of ER alpha in rat prostate results in primarily nuclear staining. |
| Localization: | Nuclear |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| Application Summary: | immunohistochemistry-Fr, Western Blot |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.05% sodium azide |
| General References: | General / background references:Kuiper GG et al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology 138:863-70 (1997). Traish AM & Pavao M Binding of site-directed monoclonal antibodies to an epitope located in the A/B region (amino acids 140-154) of human estrogen receptor-induced conformational changes in an epitope in the DNA-binding domain. Steroids 61:549-56 (1996). |
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