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| Catalog Code: | NB120-3557 |
| Product Name: | CAMKIV Antibody |
| Product Description: | Rabbit Polyclonal anti-CAMKIV |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic Peptide: V(127)EKGYYSERDAADAVKQ(143)C.Immunizing peptide corresponds to amino acid residues 127-143 from mouse CaMKIV. This sequence is 94% conserved in rat and human CaMKIV. |
| Specificity: | NB120-3557 detects CaM kinase IV (CaMKIV) from human, mouse, rat, canine, primate, arabidopsis and Xenopus Oocyte samples. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse, Dog and Primate.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 0.5ug/ml. Immunohistochemistry: use at a concentration of 1ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C / EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 0.5 µg/ml, Immunohistochemistry-Frozen 1 µg/ml |
| Species Summary: | Ca, Hu, Mu, Mk, Rt, Pl, Xp, |
| Alternate Names: | anti-Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody, anti-Brain Ca(2+) calmodulin dependent protein kinase type IV antibody, anti-Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody, anti-Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody, anti-Calcium/calmodulin dependent protein kinase IV antibody, anti-Calcium/calmodulin dependent protein kinase type IV antibody, anti-CAM kinase 4 antibody, anti-CAM kinase GR antibody, anti-CAM kinase IV antibody, anti-CAMK GR antibody, anti-CaMK IV antibody, anti-CaMK4 antibody, anti-CaMKGR antibody, anti-MGC36771 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Krebs J Calmodulin-dependent protein kinase IV: regulation of function and expression. Biochim Biophys Acta 1448:183-9 (1998). |
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