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| Catalog Code: | NB120-3535 |
| Product Name: | RbAp46 Antibody |
| Product Description: | Rabbit Polyclonal anti-RbAp46 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: MASKEMFEDTVEEC, corresponding to amino acids 1-13 of Human RbAp 46. |
| Specificity: | NB120-3535 detects retinoblastoma protein (Rb) associated protein 46 (RbAp46) from human cells. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Chicken (84% identity with immunogen), Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (92% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.5 ug/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47. 6 kDa). Can be blocked with RbAp 46 peptide. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear. |
| Background: | Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms? tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry 1.0 ug/ml, immunofluorescence, Western Blot 0.5 ug/ml, Immunocytochemistry, |
| Species Summary: | Hu, |
| Alternate Names: | anti-Chromatin assembly factor 1 subunit C antibody, anti-Chromatin assembly factor I p48 subunit antibody, anti-G1/S transition control protein binding protein RbAp46 antibody, anti-Histone acetyltransferase type B subunit 2 antibody, anti-Histone binding protein RBBP 7 antibody, anti-Histone binding protein RBBP7 antibody, anti-MGC138867 antibody, anti-MGC138868 antibody, anti-MGC53418 antibody, anti-MSI1 protein homolog antibody, anti-Nucleosome remodeling factor subunit RBAP46 antibody, anti-RbAp 46 antibody, anti-RBBP 7 antibody, anti-RBBP7 antibody, anti-Retinoblastoma binding protein 7 antibody, anti-Retinoblastoma binding protein p46 antibody, anti-Retinoblastoma binding protein p48 antibody, anti-Retinoblastoma binding protein RbAp46 antibody, anti-Retinoblastoma protein associated protein 46 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide, 1mg/ml BSA |
| General References: | General / background references:Guan LS et al. Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition. J Biol Chem 273:27047-50 (1998). Brehm A et al. Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature 391:597-601 (1998). Qian YW & Lee EY Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast. J Biol Chem 270:25507-13 (1995). |
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