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| Catalog Code: | NB120-3532 |
| Product Name: | Prohormone Convertase 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Prohormone Convertase 1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic Peptide: S(111)VQKDSALDLFNDPMWN(127). Synthetic peptide corresponds to amino acid residues 111-127 from mouse PC1. This sequence is 88% and 82% conserved bovine and rat, respectively. |
| Cross Reactivity: | Cross-reacts with Mouse. Expected to cross-react with Rat and Cow due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Western Blot: use at a concentration of 0.4 ug/ml. By Western blot, this antibody detects an ~80 kDa protein representing PC1 from mouse pituitary extract. |
| Background: | The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| Application Summary: | Western Blot |
| Species Summary: | Mu, |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% sodium azide |
| General References: | General / background references:Wang J et al. The prohormone convertase enzyme 2 (PC2) is essential for processing pro-islet amyloid polypeptide at the NH2-terminal cleavage site. Diabetes 50:534-9 (2001). Furuta M et al. Severe defect in proglucagon processing in islet A-cells of prohormone convertase 2 null mice. J Biol Chem 276:27197-202 (2001). Muller L et al. Processing and sorting of the prohormone convertase 2 propeptide. J Biol Chem 275:39213-22 (2000). |
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