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| Catalog Code: | NB120-3530 |
| Product Name: | Calcium Pump Antibody |
| Product Description: | Rabbit Polyclonal anti-Calcium Pump |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic Peptide: A(5)NSSIEFHPKPQQQREVP(22). Immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA 3 ATPase protein. This sequence is completely conserved between rat and human PMCA 3 ATPase. |
| Specificity: | Detects PMCA 3a and 3b ATPase. |
| Cross Reactivity: | Cross-reacts with Rat.Expected to cross-react with Human due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a dilution of 1/1000. ELISA: assay dependent. Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~127 kDa protein representing PMCA 3a and PMCA 3b ATPase from rat brain microsomal fractions. |
| Background: | The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | ELISA , Western Blot 1:1,000 |
| Species Summary: | Rt |
| Alternate Names: | anti-ATP2B1 antibody, anti-ATPase Ca++ transporting plasma membrane 1 antibody, anti-Plasma membrane calcium ATPase 1 antibody, anti-Plasma membrane calcium ATPase isoform 1 antibody, anti-Plasma membrane calcium pump isoform 1 antibody, anti-Plasma membrane calcium transporting ATPase 1 antibody, anti-PMCA 1 antibody, anti-PMCA1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | This product has been used in:Filoteo AG et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-7 (1997). General / background references:Caride AJ et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J 316 (Pt 1):353-9 (1996). |
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