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| Catalog Code: | NB120-3516 |
| Product Name: | Calsequestrin Antibody |
| Product Description: | Rabbit Polyclonal anti-Calsequestrin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Purified canine cardiac Calsequestrin. |
| Specificity: | This antibody detects Calsequestrin. It recognizes both cardiac and skeletal muscle Calsequestrin. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse, Dog and Sheep. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | Western Blot, ImmunoprecipitationNot tested in other applications. By Western blot, this antibody detects an ~55 kDa protein representing Calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be Calsequestrin-like proteins. |
| Localization: | Isoform 1: Sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial Isoform 2: Smooth endoplasmic reticulum. |
| Background: | The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium. Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunoprecipitation Assay dependent, Western Blot 1/2500 |
| Species Summary: | Ca, Hu, Mu, Rt, Sh, |
| Alternate Names: | anti-Calmitin antibody, anti-Calmitine antibody, anti-Calsequestrin 1 antibody, anti-Calsequestrin 1 fast twitch skeletal muscle antibody, anti-Calsequestrin 2 antibody, anti-Calsequestrin 2 fast twitch cardiac muscle antibody, anti-Calsequestrin cardiac muscle isoform antibody, anti-Calsequestrin skeletal muscle isoform antibody, anti-Calsequestrin1 antibody, anti-Calsequestrin2 antibody, anti-Cardiac calsequestrin 2 antibody, anti-Cardiac calsequestrin antibody, anti-CASQ 1 antibody, anti-CASQ 2 antibody, anti-CASQ antibody, anti-CASQ1 antibody, anti-CASQ2 antibody, anti-CSQ1 antibody, anti-FLJ26321 antibody, anti-PDIB 1 antibody, anti-PDIB 2 antibody, anti-PDIB1 antibody, anti-PDIB2 antibody, anti-Skeletal muscle calsequestrin 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% Sodium Azide |
| General References: | This product has been used in:Mahony L & Jones LR Developmental changes in cardiac sarcoplasmic reticulum in sheep. J Biol Chem 261:15257-65 (1986). General / background references:Kobayashi YM & Jones LR Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium. J Biol Chem 274:28660-8 (1999). Zhang L et al. Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane. J Biol Chem 272:23389-97 (1997). |
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