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| Catalog Code: | NB120-3485 |
| Product Name: | PPAR alpha Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PPAR alpha [Ser21] |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: LEADDLESpPLSE, corresponding to amino acids 14-25 of Mouse PPAR alpha. |
| Specificity: | NB120-3485 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S21 from mouse tissues. |
| Cross Reactivity: | Cross-reacts with Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 2 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a ~52 kDa protein which corresponds to phospho PPAR alpha S21 from mouse adipose tissue extract. |
| Localization: | Nuclear |
| Background: | Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the phospho-PPAR alpha is NR1C1. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 2 ug/ml |
| Species Summary: | Mu |
| Alternate Names: | anti-peroxisome proliferator activated receptor alpha antibody, anti-PPAR-alpha antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| Phosphorylated: | Ser21 |
| General References: | General / background references:Lavinsky RM et al. Diverse signaling pathways modulate nuclear receptor recruitment of N-CoR and SMRT complexes. Proc Natl Acad Sci U S A 95:2920-5 (1998). Kilgore MW et al. MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol Cell Endocrinol 129:229-35 (1997). |
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