| CatalogCode: | | NB120-3450 |
| ProductName: | | Mint 3 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Mint 3 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: MEFLPEPQHPPGPPTMDLE, corresponding to amino acids 1-19 of Rat Mint3. |
| Specificity: | | NB120-3450 detects munc-18 interacting protein 3 (Mint3) from mouse cells and rat brain samples. |
| CrossReactivity: | | Cross-reacts with Rat and Mouse.Not yet tested in other species. |
| Packaging: | | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | | Western Blot: use at a concentration of 2 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract. |
| Background: | | The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer?s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer?s disease. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Rt, Mu |
| ProteinTarget: | | Mint 3 |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | General / background references:Nakajima Y et al. Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain. Brain Res Mol Brain Res 92:27-42 (2001). Okamoto M et al. Amyloid precursor protein associates independently and collaboratively with PTB and PDZ domains of mint on vesicles and at cell membrane. Neuroscience 104:653-65 (2001). Tanahashi H & Tabira T X11L2, a new member of the X11 protein family, interacts with Alzheimer's beta-amyloid precursor protein. Biochem Biophys Res Commun 255:663-7 (1999). |
| more information: | | company product webpage |