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| Catalog Code: | NB120-3447 |
| Product Name: | NFAT 3 Antibody |
| Product Description: | Rabbit Polyclonal anti-NFAT 3 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: CR(887)DLSGFPAPPGEEPPA(902). Immunizing peptide corresponds to amino acid residues 887-902 of human NFAT 3 protein. This sequence is 94% conserved with mouse NFAT 3. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Immunohistochemistry: use at a dilution of 1/3000.Western Blot: use at a dilution of 1/10000.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~150 kDa protein representing NFAT 3 in HEK293 cells transfected with the human NFAT 3 gene.Immunohistochemical staining of NFAT 3 in HEK293 cells transfected with the human NFAT 3 gene results in cytoplasmic staining. |
| Localization: | Cytoplasmic and Nuclear. |
| Background: | The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry 1:3,000, Western Blot 1:10,000 |
| Species Summary: | Hu |
| Alternate Names: | anti-Cytoplasmic nuclear factor of activated T cells 4 antibody, anti-NF AT3 antibody, anti-NF ATc4 antibody, anti-NFAT 3 antibody, anti-NFAT C4 antibody, anti-NFATC 4 antibody, anti-NFATC4 antibody, anti-Nuclear Factor of Activated T cells antibody, anti-Nuclear factor of activated T cells cytoplasmic 4 antibody, anti-Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4 antibody, anti-T cell transcription factor NFAT 3 antibody, anti-T cell transcription factor NFAT3 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Shen K & Meyer T Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Science 284:162-6 (1999). |
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