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| Catalog Code: | NB120-3439 |
| Product Name: | Serotonin N-acetyltransferase Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Serotonin N-acetyltransferase [Thr29] |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: C(24)QRRHT(p)LPAS(33).Immunizing phosphopeptide corresponds to N-terminal amino acid residues 24-33 of mouse AA-NAT.This sequence is completely conserved between mouse, rat, and human AA-NAT protein. |
| Specificity: | NB120-3439 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues. |
| Cross Reactivity: | Cross-reacts with Rat and Mouse.Expected to cross-react with Human due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 0.5 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract. |
| Background: | Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 0.5 µg/ml |
| Species Summary: | Rt, Mu |
| Package Size: | 0.05 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| Phosphorylated: | Thr29 |
| General References: | General / background references:Li X et al. Molecular rhythms in the pineal gland. Curr Opin Neurobiol 8:648-51 (1998). |
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