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| Catalog Code: | NB120-3433 |
| Product Name: | Transducin beta Antibody |
| Product Description: | Rabbit Polyclonal anti-Transducin beta |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic Peptide: R(8)QEAEQLKNQIRDARKAC(25). Immunizing peptide corresponds to amino acid residues 8-25 from human Transducin beta. This sequence is completely conserved between human, amphibian, bovine and rat. |
| Specificity: | The NB120-3433 immunizing peptide corresponds to amino acid residues 8-25 from human Tr beta. This sequence is completely conserved between human, amphibian, bovine and rat. |
| Cross Reactivity: | Cross-reacts with Human and Sheep.Expected to cross-react with Rat, Cow and Amphibians due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 1 ug/ml.Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~37 kDa protein representing Transducin beta from sheep retinal extract. |
| Background: | Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 2 µg/ml |
| Species Summary: | Hu, Sh |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Calvert PD et al. Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A 97:13913-8 (2000). Baylor D How photons start vision. Proc Natl Acad Sci U S A 93:560-5 (1996). |
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