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| Catalog Code: | NB120-3431 |
| Product Name: | SCAMP 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-SCAMP 2 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic Peptide: M(1)SAFDTNPFADPVDVNP(17)C. Immunizing peptide corresponds to amino acid residues 1-17 from human SCAMP 2.This sequence is completely conserved between human and mouse. |
| Specificity: | Detects Secretory Carrier Membrane Protein 2 (SCAMP 2) from mouse testes and AtT20 cell extract, as well as Chinese hamster ovary (CHO) cell extract. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Hamster.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 2 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~38 kDa protein representing SCAMP 2 from CHO cell extract. |
| Background: | Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 2 µg/ml |
| Species Summary: | Ha, Hu, Mu |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Windoffer R et al. Tissue expression of the vesicle protein pantophysin. Cell Tissue Res 296:499-510 (1999). Wu TT & Castle JD Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor. Mol Biol Cell 9:1661-74 (1998). Singleton DR et al. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. J Cell Sci 110 Pt 17:2099-107 (1997). |
| Novus References: | Jain, P., et al.Identification of Human T Cell Leukemia Virus Type 1 Tax Amino Acid Signals and Cellular Factors Involved in Secretion of the Viral Oncoprotein. J. Biol. Chem., Nov 2007; 282:34581-34593. |
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