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| Catalog Code: | NB120-3423 |
| Product Name: | Resistin Antibody |
| Product Description: | Rabbit Polyclonal anti-Resistin |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: SSMPLCPIDEAIDKKIKQDC, corresponding to amino acids 21-39 of Mouse Resistin. |
| Specificity: | Detects recombinant mouse resistin. |
| Cross Reactivity: | Cross-reacts with Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 2 ug/ml.Not tested in other applications.Optimal working dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~14 kDa protein representing recombinant mouse resistin. |
| Localization: | Secreted |
| Background: | Adipose tissue is an energy reserve in animals and, in nondomestic species, is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 2 µg/ml |
| Species Summary: | Mu |
| Alternate Names: | anti-Adipose tissue specific secretory factor antibody, anti-ADSF antibody, anti-C/EBP epsilon regulated myeloid specific secreted cysteine rich protein antibody, anti-Cysteine rich secreted protein A12 alpha like 2 Cysteine rich secreted protein FIZZ3 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Sentinelli F et al. Human resistin gene, obesity, and type 2 diabetes: mutation analysis and population study. Diabetes 51:860-2 (2002). Way JM et al. Adipose tissue resistin expression is severely suppressed in obesity and stimulated by peroxisome proliferator-activated receptor gamma agonists. J Biol Chem 276:25651-3 (2001). Steppan CM et al. The hormone resistin links obesity to diabetes. Nature 409:307-12 (2001). |
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