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| Catalog Code: | NB120-3420 |
| Product Name: | PICK 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-PICK 1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: MFADLDYDIEEDKLGIPT, corresponding to amino acids 1-18 of Mouse PICK1. |
| Specificity: | Detects protein interacting with Protein Kinase C (PICK 1). |
| Cross Reactivity: | Cross-reacts with Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Immunocytochemistry: use at a concentration of 1.7 ug/ml. Western Blot: use at a concentration of 2 ug/ml. Immunoprecipitation: assay dependent. By Western blot, this antibody detects an ~52 kDa protein representing PICK 1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7. |
| Localization: | Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses. |
| Background: | PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| Application Summary: | immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Mu, Rt, |
| Alternate Names: | anti-PICK1 antibody, anti-protein interacting with PRKCA 1 antibody, anti-MGC15204 antibody, anti-PRKCABPantibody, anti-OTTHUMP00000028509 antibody, anti-PRKCA-binding protein antibody, anti-protein interacting with C kinase 1 antibody, anti-protein kinase C antibody, anti-alpha binding protein antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% sodium azide |
| General References: | General / background references:Perez JL et al. PICK1 targets activated protein kinase Calpha to AMPA receptor clusters in spines of hippocampal neurons and reduces surface levels of the AMPA-type glutamate receptor subunit 2. J Neurosci 21:5417-28 (2001). Staudinger J et al. Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-alpha. J Biol Chem 272:32019-24 (1997). |
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