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| Catalog Code: | NB120-3341 |
| Product Name: | Ubiquilin Antibody |
| Product Description: | Rabbit Polyclonal anti-Ubiquilin |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: AESAESGGPPGAQDSAA, corresponding to amino acids 2-18 of Mouse Ubiquilin. |
| Cross Reactivity: | Cross-reacts with Mouse and Hamster.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Western Blot: use at a concentration of 1 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
| Background: | Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins are nicastrin and ubiquilin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Ubiquilin is believed to promote presenilin protein accumulation. Also found in the neurofibrillary lesions associated with Alzheimer's disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer's diseased patients. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 1 µg/ml |
| Species Summary: | Ha, Mu |
| Alternate Names: | anti-DA 41 antibody, anti-DA41 antibody, anti-DSK 2 antibody, anti-DSK2 antibody, anti-FLJ90054 antibody, anti-hPLIC-1 antibody, anti-hPLIC1 antibody, anti-PLIC-1 antibody, anti-PLIC1 antibody, anti-Protein linking IAP with cytoskeleton-1 antibody, anti-Ubiquilin 1 antibody, anti-Ubiquilin1 antibody, anti-UBQLN 1 antibody, anti-UBQLN1 antibody, anti-XDRP 1 antibody, anti-XDRP1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | General / background references:Conklin D et al. Molecular cloning, chromosome mapping and characterization of UBQLN3 a testis-specific gene that contains an ubiquitin-like domain. Gene 249:91-8 (2000). Mah AL et al. Identification of ubiquilin, a novel presenilin interactor that increases presenilin protein accumulation. J Cell Biol 151:847-62 (2000). |
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more information: | company product webpage |