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| Catalog Code: | NB120-3306 |
| Product Name: | Chk 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Chk 1 (DCS-300) |
| Clone: | DCS-300 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Recombinant full length protein (Human). |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | Western Blotting: use at a concentration of 5 - 10ug / ml for 2hrs at RT. By Western blot, this antibody detects a band of 56 kDa, which corresponds to the predicted molecular weight of Chk 1. |
| Localization: | Nuclear. |
| Background: | One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2b kappa |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| Application Summary: | Western Blot 5 - 10ug/ml |
| Species Summary: | Hu, |
| Alternate Names: | anti-Cell cycle checkpoint kinase antibody; anti-CHEK1 antibody; anti-Serine/threonine protein kinase Chk1 antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
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more information: | company product webpage |